A way of identifying
DNA fragments that have been cut with
restriction enzymes (usually after they've been run on an
electrophoresis gel) by eluting them onto a
nitrocellulose filter or some other solid support, and then exposing them to
radioactive or
fluorescent DNA probes
complementary to the sequences known or expected to be on the fragments being identified. The radioactive bands are then used to expose film, which will tell you where your DNA fragments were on the gel. In most setups, their position on the gel is directly related to the number of
bases they have.
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